In the present research, a higher level of microbial adherence was observed to braided threads g2, g3, and g4, in agreement with previous studies 11, 12. Pdf bacterial adherence around sutures of different. G1 nylon, g2 silk, g3 polyglactin 910, g4 polyglactin 910 with triclosan. Further observation was also conducted between the study groups different suture materials by analyzing the bacterial load. It was shown that any suture material can host or harbor bacterial biofilm formation, but not all suture materials are necessarily equivalent in this regard. Triclosancoated and uncoated suture materials were applied in 17 patients undergoing wisdom tooth extraction. Bacterial colonization on different suture materialsa. N ew suture materials and designs are constantly being introduced to the surgical marketplace. Four different suture materials were used to suture the nasal septum. Upon flap closure, four different sutures were placed in a randomized sequencesilk, coated polyglactin, nylon, and polyester. The zois around triclosancoated suture were significantly greater for s pseudintermedius isolates than for e coli isolates. Intragroup comparison of microbial aerobic and anaerobic colonyforming units cfuml on various intraoral suture materials with silk from 60 patients who were divided into 5 groups of 12. Wound closure in particular is, therefore, prone to move bacteria from the skin into deeper tissue layers, carried by the suture material.
Bacterial adherence to hightensile strength sutures. Pdf microbial adherence on various intraoral suture. However, bacteria colonization is not related only with the type of the suture material but also with bacteria immigration from penetration holes of suture. Microbial adherence on various intraoral suture materials in. Suture material is an operator dependent variable and while little objective data exist to guide the choice of suture, it may play a role in wound infection. Adherence of bacteria to sutures may be an integral part of the pathogenesis of certain surgical infections. This study investigated the adherence of oral microorganisms to different types of suture threads. Every suture material is capable, albeit not equally, of holding bacterial biofilm formation, which can be a source of surgical site infection. Patients scheduled for implant or periodontal surgery were included in the study.
The techniques of wound management and closure are wellcovered as a part of any em rotation, but this often focuses on the technical aspects of irrigation and closure. We expected bacterial growth on the barbed suture material to be reduced in comparison to polyfilament sutures, but increased when compared with monofilament sutures. A microbiological analysis article pdf available in materials 1218. Investigations were concentrated on the total number of viable bacteria and the spectrum of aerobes facultative anaerobes and strict anaerobes on the two suture materials. Orthocord suture features high tensile and knot strength balance of knot slide ability, knot security, stiffness and elastic tension less likelihood of abrasion from surgical anchors, surrounding tissues or surgeons hands1 small knot pro. The effects of different suture materials in the nasal cavity. Prevention of bacterial colonization on nonthermal. Bacterial adhesion to synthetic materials such as suture threads, prostheses, and grafts have been the objects of study of many researchers 1, 11 12. Staphylococcus aureus adhered to suture material and formed a structure consistent with a bacterial biofilm. This study used a standardized in vitro microbiologic model to assess bacterial adherence and the antibacterial activity of a triclosancoated polyglactin 910 braided suture against selected grampositive and gramnegative clinical isolates that may infect surgical wounds. Triclosancoated sutures do not reduce leg wound infections. We undertook this study to compare the bacterial adherence in vitro to. Scanning electron microscopy was performed to examine qualitatively the level of bacterial adherence.
Monofilament and barbed suture have been shown in invitro models to have less bacterial adherence than a braided suture. An antibacterial suture material with enhanced wound healing function may. Microbial adherence on 2 different suture materials in. Silk and cotton sutures are the most commonly used materials for skin closure, the choice being largely based on tradition.
Tissue reactions to suture materials used for oral surgical interventions may vary depending on the surface properties and bacterial adherence properties of the material. This study evaluates bacterial adherence to suture materials and tissue reactivity with a bioluminescent invivo mouse model. The comparison of microbial adherence to various sutures in patients undergoing oral surgery the safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Request pdf bacterial adherence to suture materials wound infections may be problematic for physicians. A variety of suture materials are available for primary wound closure following oral surgical procedures. Though very less anaerobic bacteria growth was seen among all tested suture materials, it was maximum around vicryl and polyamide sutures. Colonization by pathogens on sutures leads to the recommendation. The effects of suture materials on intranasal encrustation were evaluated with anterior rhinoscopy. Comparison of antibacterialcoated and noncoated suture material in intraoral surgery by isolation of adherent bacteria routine wound treatment during dentoalveolar surgery. Can antibacterialcoated sutures reduce the risk of microbial contamination. Bacterial adhesion to suture material in a contaminated wound model. Laceration repair is a common procedure in the emergency department ed. Antibacterial sutures for wound closure after surgery.
Bacterial adherence to hightensile strength sutures may be a useful factor in implant selection in a patient with predisposition for contamination or infection. Objective to evaluate adherence of methicillinresistant staphylococcus pseudintermedius mrsp to 5 suture materials commonly used in small animal surgery sample 10 epidemiologically unrelated mrsp isolates obtained from dogs with clinical infections that had strong biofilmforming ability and 5 types of suture. Pieces of thread were distributed on 24well plates, according to the following groups. Hence, this study investigated different suture materials used at surgical sites and their contribution to surgical site infection. The degree of infection obtained in mice in the presence of different sutures nicely correlated with their adherence properties. Adherence of bacteria to suture materials sage journals. The bacterial adherence for both organisms at all time intervals was significantly greater to silk than to cotton, except at 60 hours for staphylococcus aureus. The vicryl suture had the highest counts and was statistically significant in bacterial adherence versus all other sutures. Ten days following surgery, the sutures were removed and incubated in. Microbial adherence on various intraoral suture materials in patients undergoing dental surgery. Vicryl plus is a polyglacitin suture coated with the antiseptic chemical substance triclosan, which has been shown to inhibit the growth of staphylococcus aureus in vitro. Sep 04, 2019 results were analyzed statistically using the anovat test, which showed that the difference between aerobic and anaerobic bacterial adherence around different suture materials within the group was statistically significant.
We report the findings of a prospective study into the bacterial contamination of monofilament nylon sutures removed from corneal wounds. The aim of the study was to compare bacterial accumulation on different suture materials following oral surgery. Conclusion polypropylene suture material showed significantly lower bacterial adherence and superior clinical features compared to silk, including better soft tissue healing. Bacterial adherence to uncoated polyglactin910 was greatest, followed by triclosancoated polyglactin910, and then uncoated monofilament sutures, with least adherence to coated monofilament sutures. Bacterial adherence around sutures of different material at.
Pgc25 suture has a lower bacteria adherence rate than pgaand silk. Selected suture samples were prepared for scanning electron micros copy to demonstrate bacterial adherence. Bacterial contamination of nylon corneal sutures eye. Bacterial adherence to suture materials sciencedirect. Bacterial adherence to suture materials request pdf. The barbed monofilament suture showed the least bacterial adherence of any suture material tested. Bacteria adhere less to barbed monofilament than braided. The colonization rate of streptococcus intermedius on both sutures was similar. Absorbable silk and monocryl exhibited the smallest number of adherent bacteria.
Suture materials primary purpose for suture approximate wound margins enhance tissue healing early role of suture wounds do not gain strength until 46 days after injury approximating of tissues depending on suture strength the relationship between gradual loss of suture strength and slow increase of wound strength must be. We report the findings of a prospective study into the bacterial contamination of monofilament nylon sutures removed from corneal wounds following cataract surgery. Bacterial adherence to vascular prostheses journal of. Bacterial adherence around sutures of different material at grafted. The choice of suture material is a surgeondependent variable. The comparison of microbial adherence to various sutures in. Decreased bacterial adherence, biofilm formation, and tissue. Bacterial adherence around sutures of different material at grafted site. Materials free fulltext bacterial adherence around.
This study evaluates bacterial adherence to suture materials and tissue reactivity with an in vivo contaminated wound mouse model. May 21, 2016 to reinvestigate, we used a comparable experimental design to semiquantitatively measure bacterial growth on different suture materials in a contaminated wound model. The growth of prevotella intermedia was much better on deknalon than on. Selected suture samples were prepared for scanning electron microscopy to demonstrate bacterial adherence. J oral maxillofac surg microbial adherence on various. This study evaluates bacterial adherence to commonly used suture materials with a bioluminescent in vitro model. The bacterial count for each suture material appeared to be an intrinsic property of the suture and did not vary with the concentration. In intraoral surgery, sutures are faced with different bacterial species and the question arises whether the antibacterialcoated suture material has the same positive effects. Bacterial adherence to a suture material is one of the main reasons that cause surgical site infections.
Sep 22, 2012 to determine adherence, we determined the number of bacteria removed from the suture by sequential washes. Bacterial adherence to surgical sutures semantic scholar. Leg wound infection is a common complication after coronary artery bypass grafting cabg. The concept of the relative hydrophobicity of biomaterial and its influence on bacterial adhesion has been demonstrated in studies of bacterial adherence to suture material 21 x 21 katz, s, izhar, m, and mirelman, d. The different removal rate of adherent bacteria glutaraldehydefixed from various sutures by the tissue factors in mice supports the hypothesis that bacterial adherence to suture materials plays a significant role in the induction of surgical infection. Bacterial adhesion to suture material in a contaminated wound. To investigate the effects of different suture materials in the nasal cavity on encrustation and microorganism colonisation. Bacterial suture adherence and biofilm formation in an in.
Our observation points out at the need for careful suture selection in contaminated wounds. Suture contamination has been suggested as a mechanism of surgical site infections. Microbial accumulation on different suture materials. Tissue reactions to various suture materials used in oral. Pdf microbial adherence on various intraoral suture materials in. Sem investigation of bacteria adhesion on polyglecaprone 25. Staphylococcus aureus was used to create an in vivo contaminated wound model at 2 amounts 106 colonyforming units cfu and 108 cfu using a mouse air pouch.
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